We have 2 separate labs: a muscle physiology lab and a biochemistry lab. The muscle physiology lab is approximately 1000 sq. ft. of space that has been recently renovated for the study of muscle contractile properties, animal surgeries, and tissue handling/storage. It contains areas for the set-up of in vivo, in situ, in vitro, and single fiber isolation of muscle.
The equipment in the muscle physiology lab is as follows: 3 muscle lever systems (Cambridge) and transducers ( 0-5000 g range - 0-50 g range - 0-100 mg range) for the in vivo and in situ, in vitro, and single fiber preps, respectively, helium-neon laser, isolated organ baths (3), stimulator and isolation unit, motorized micromanipulators (2), 6-channel physiographs (2), pressure transducers, computer and data acquisition system (3), small animal ventilator, rapid freezing biopsy drill, freeze drier, electronic balance (2), pH meter, dissolved oxygen meter, peristaltic pump, distiller, refrigerator, fume hood, tissue homogenizing equipment, a low temperature freezer (-80 C), refrigerator (0-5 C), and standard freezer (-20 C), a liquid nitrogen dewar, pH meters (2), a dissecting microscope, power supply and gel boxes (6,) compound microscope, microhematocrit centrifuge, analytical balances (2), cryostat and an isoflurane anesthesia cart.
The above laboratory has a combined total of 3 computers. Two computers are interfaced with laboratory equipment and dedicated to data collection. One is free-standing and used primarily for statistical analysis.